Fig. 4

Regulatory element features of the region overlapped by the differentially methylated cytosine DMC23, which was correlated with GNAS expression, in adipose (A) and muscle (M). DMC23 (represented by the vertical black line) overlapped a CpG island (CpG358, represented by the dark green solid horizontal bar) in the intron 1 of GNAS isoform 001,271,771 and less than 1 kb from the start site of isoform 181,021. Histone marks and ATAC peaks enrichment suggested that this region was classified as bivalent/poised TSS (represented by the orange solid horizontal bar #12) in muscle (M) and strongly active promoter/transcript in adipose (A; red solid horizontal bar #1) in two male Bos taurus [23]. The GNAS gene is represented by the blue line (introns) and blue blocks (exons). Pink tracks represent the ATAC peaks. The peaks of histone marks H3K27ac, H3K27me, H3K4me1 and H3K4me3 are represented by the red, black, yellow, and green tracks, respectively. The image was obtained from UCSC Genome Browser and edited by the authors