Fig. 2From: Histone H3.3 K27M chromatin functions implicate a network of neurodevelopmental factors including ASCL1 and NEUROD1 in DIPGH3.3K27M DIPG cells are enriched for open chromatin peaks in upregulated genes related to the nervous system compared to their isogenic gene-edited wild-type counterparts. a Bar plot showing proportion of peaks in various genomic regions. Promoters were defined as 2 kb upstream of the TSS and 1 kb downstream. 75% or more of the peaks for each line were found in intergenic regions or introns. b H3K4me3 levels at genes associated with changes in ATAC-seq peaks between Line XIII PAR (K27M, blue) and WT (wild-type, red) cell lines, validated by ChIP–qPCR. n = 2 biological replicates, *p value < 0.05, **p value < 0.01, ***p value < 0.001 and error bars were calculated based on standard deviation. c Gene ontology analysis of differential open chromatin (left side) and specifically open chromatin regions linked to increased gene expression (right side) in XIII, d XIII-WT, and e XVII. BP biological processes, CC cellular components, KEGG KEGG Pathways, and MF molecular function. For enriched GO terms p values were obtained from the Benjamini–Hochberg methodBack to article page