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Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: Pioneer transcription factors are associated with the modulation of DNA methylation patterns across cancers

Fig. 5

FOXA1 endogenous interaction with TET1 and TET2. We employed endogenous IP on nuclear extracts obtained from MCF-7 cells. A GST-pulldown of FOXA1-V5 using GST-TET1. B GST-pulldown of FOXA1-V5 using GST-mTET2. C Immunoprecipitation of endogenous FOXA1 using rabbit anti-FOXA1 antibody to detect endogenous TET1 pulled together with endogenous FOXA1. D Immunoprecipitation of endogenous FOXA1 using rabbit anti-FOXA1 antibody and detecting endogenous TET2 pulled together with FOXA1. Non-specific bands are marked with asterisks (*)

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Epigenetics & Chromatin

ISSN: 1756-8935