Skip to main content
Fig. 6 | Epigenetics & Chromatin

Fig. 6

From: Pan-cancer predictions of transcription factors mediating aberrant DNA methylation

Fig. 6

Loss of DNA methylation upon GATA3 overexpression in hTERT-HME1 normal cells. a Western blot analysis of GATA3 protein levels in HCC1954 cells and hTERT-HME1 cells stably transfected with a plasmid containing the GATA3 ORF (HME1-GATA3) or non-transfected (HME1-WT). GAPDH was used as a control for equal loading. b ChIP-qPCR analysis for GATA3 binding at two positive control regions CR1 and CR2 (regions unmethylated in hTERT-HME1 and HCC1954 cells with GATA3 motif and binding in HCC1954 cells), at two regions of interest ROI1 and ROI2 from Fig. 5f (regions methylated in hTERT-HME1 cells, unmethylated in HCC1954 cells, with GATA3 motif and binding in HCC1954 cells, and gaining methylation in GATA3 KO clones), and at two negative control regions NC1 (region methylated in hTERT-HME1 cells, unmethylated in HCC1954 cells with no GATA3 motif and binding in HCC1954 cells), NC2 (intergenic region unmethylated in hTERT-HME1 and HCC1954 cells with no GATA3 motif and binding in HCC1954 cells). ChIP was performed with antibody against GATA3 or a control IgG antibody. ChIP signals are represented as percentage input. Data represent mean ± SEM (n = 3 biological replicates). c Patterns of CpG methylation of ROI1, ROI2 and NC1 by bisulfite sequencing analysis in HME1 WT and HME1 overexpressing GATA3 (HME1-GATA3). White circles represent unmethylated CpGs and black circles represent methylated CpGs

Back to article page