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Table 2 Differentially expressed genes between ICSI- and mICSI-derived blastocysts

From: Mitochondrial supplementation of Sus scrofa metaphase II oocytes alters DNA methylation and gene expression profiles of blastocysts

Gene_ida Chr/Scafoldb Start End Strand Gene_symbol Gene_biotype Description logFCc adj.P.Vald
Upregulated in mICSI-derived blastocysts relative to ICSI        
ENSSSCG00000050793 9 47,00,784 47,00,878 ssc-mir-10390 miRNA ssc-mir-10390 [Source:miRBase;Acc:MI0033404] 8.41 1.46E−08
ENSSSCG00000030088 AEMK02000569.1 7,21,734 7,43,707  +    protein_coding colony stimulating factor 2 receptor alpha subunit [Source:NCBI gene;Acc:100620339] 6.16 4.90E−02
ENSSSCG00000045022 14 7,38,21,782 7,38,28,231  +    lncRNA NULL 6.02 1.30E−02
ENSSSCG00000045442 AEMK02000694.1 25,505 52,916   protein_coding leucine carboxyl methyltransferase 1-like [Source:NCBI gene;Acc:100625764] 6.00 1.30E−02
ENSSSCG00000021584 15 12,09,85,245 12,09,91,318  +  CDK5R2 protein_coding cyclin dependent kinase 5 regulatory subunit 2 [Source:HGNC Symbol;Acc:HGNC:1776] 5.67 2.94E−02
ENSSSCG00000047552 9 7,63,60,171 7,63,60,887  +    protein_coding NULL 5.40 2.94E−02
ENSSSCG00000016816 16 1,95,47,009 1,99,20,515 ADAMTS12 protein_coding ADAM metallopeptidase with thrombospondin type 1 motif 12 [Source:HGNC Symbol;Acc:HGNC:14605] 5.16 2.94E−02
ENSSSCG00000045298 9 7,73,07,796 7,73,15,488   lncRNA NULL 4.80 4.41E−02
ENSSSCG00000051325 1 24,83,28,317 24,84,48,089   lncRNA NULL 4.31 4.96E−02
ENSSSCG00000010017 14 4,78,79,317 4,79,02,699  +  SMTN protein_coding smoothelin [Source:NCBI gene;Acc:414369] 3.56 1.41E−03
ENSSSCG00000043834 AEMK02000692.1 81,135 86,443  +    protein_coding NULL 3.46 3.82E−03
ENSSSCG00000017592 12 2,74,50,805 2,75,26,985 MBTD1 protein_coding mbt domain containing 1 [Source:HGNC Symbol;Acc:HGNC:19866] 2.93 3.72E−03
ENSSSCG00000015729 15 2,96,75,820 2,97,14,079 TSN protein_coding translin [Source:HGNC Symbol;Acc:HGNC:12379] 2.68 7.98E−04
ENSSSCG00000046732 AEMK02000589.1 1,03,902 1,10,514  +    lncRNA NULL 2.65 3.45E−02
ENSSSCG00000050138 AEMK02000256.1 1,71,630 1,81,191  +    lncRNA NULL 2.49 1.25E−03
ENSSSCG00000014362 2 14,21,02,456 14,21,15,344 HBEGF protein_coding heparin binding EGF-like growth factor [Source:HGNC Symbol;Acc:HGNC:3059] 2.29 1.49E−02
ENSSSCG00000031712 5 6,27,42,315 6,27,59,190  +  MFAP5 protein_coding microfibril associated protein 5 [Source:HGNC Symbol;Acc:HGNC:29673] 2.06 2.94E−02
ENSSSCG00000007043 17 1,44,20,656 1,44,76,189 GPCPD1 protein_coding glycerophosphocholine phosphodiesterase 1 [Source:HGNC Symbol;Acc:HGNC:26957] 1.79 4.18E−02
ENSSSCG00000015604 9 13,15,47,084 13,15,60,527  +  NEK2 protein_coding NIMA related kinase 2 [Source:HGNC Symbol;Acc:HGNC:7745] 1.57 4.90E−02
ENSSSCG00000011704 13 8,97,42,893 8,98,89,187 WWTR1 protein_coding WW domain containing transcription regulator 1 [Source:HGNC Symbol;Acc:HGNC:24042] 1.33 3.29E−02
ENSSSCG00000015144 9 5,02,58,971 5,05,09,799  +  GRAMD1B protein_coding GRAM domain containing 1B [Source:HGNC Symbol;Acc:HGNC:29214] 1.28 2.55E−02
ENSSSCG00000020927 13 10,95,87,256 10,96,23,787 SLC2A2 protein_coding solute carrier family 2 member 2 [Source:NCBI gene;Acc:397429] 1.26 9.43E−03
ENSSSCG00000034282 15 11,72,44,763 11,74,51,261 ABCA12 protein_coding ATP binding cassette subfamily A member 12 [Source:HGNC Symbol;Acc:HGNC:14637] 1.09 4.96E−02
ENSSSCG00000030211 12 1,97,55,737 1,97,88,952 NBR1 protein_coding NBR1 autophagy cargo receptor [Source:HGNC Symbol;Acc:HGNC:6746] 1.07 1.67E−02
Downregulated in mICSI-derived blastocysts relative to ICSI        
ENSSSCG00000018062 MT 2206 2273  +    Mt_tRNA product = tRNA-Val − 11.31 4.32E−06
ENSSSCG00000018070 MT 6129 6196  +    Mt_tRNA product = tRNA-Trp − 8.93 1.34E−04
ENSSSCG00000018079 MT 8891 8957  +    Mt_tRNA product = tRNA-Lys − 8.81 2.02E−05
ENSSSCG00000018073 MT 6379 6444   Mt_tRNA product = tRNA-Cys − 8.30 1.55E−04
ENSSSCG00000018071 MT 6203 6270   Mt_tRNA product = tRNA-Ala − 7.55 8.92E−04
ENSSSCG00000032749 8 8,93,21,491 8,93,35,395  +  PCDH18 protein_coding protocadherin 18 [Source:HGNC Symbol;Acc:HGNC:14268] − 6.48 9.43E−03
ENSSSCG00000018072 MT 6272 6346   Mt_tRNA product = tRNA-Asn − 6.10 6.78E−03
ENSSSCG00000012257 X 3,89,29,231 3,90,06,222  +    protein_coding monoamine oxidase A [Source:NCBI gene;Acc:414424] − 5.94 3.09E−02
ENSSSCG00000051497 5 10,21,87,453 10,27,20,929   lncRNA NULL − 5.77 7.62E−03
ENSSSCG00000021343 15 74,99,026 76,32,655  +  ZEB2 protein_coding zinc finger E-box binding homeobox 2 [Source:HGNC Symbol;Acc:HGNC:14881] − 5.56 3.45E−02
ENSSSCG00000050427 2 12,87,99,599 12,92,30,191   lncRNA NULL − 5.53 2.38E−02
ENSSSCG00000001097 7 1,95,87,685 1,98,38,093   protein_coding RHO family interacting cell polarization regulator 2 [Source:NCBI gene;Acc:100154661] − 5.35 2.12E−02
ENSSSCG00000037634 7 6,24,71,228 6,24,79,223  +  FOXA1 protein_coding forkhead box A1 [Source:HGNC Symbol;Acc:HGNC:5021] − 5.18 3.45E−02
ENSSSCG00000001484 7 2,64,46,306 2,65,22,965 TINAG protein_coding tubulointerstitial nephritis antigen [Source:HGNC Symbol;Acc:HGNC:14599] − 5.14 4.87E−02
ENSSSCG00000045069 7 1,68,85,827 1,68,91,770   lncRNA NULL − 4.96 4.56E−02
ENSSSCG00000018074 MT 6444 6509   Mt_tRNA product = tRNA-Tyr − 4.73 2.94E−02
ENSSSCG00000035347 14 10,60,13,495 10,67,13,121  +  CYP2C42 protein_coding cytochrome P450 C42 [Source:NCBI gene;Acc:403111] − 3.95 3.31E−03
ENSSSCG00000015780 15 4,50,83,606 4,53,01,158  +  STOX2 protein_coding storkhead box 2 [Source:HGNC Symbol;Acc:HGNC:25450] − 3.41 2.94E−02
ENSSSCG00000023204 3 4,13,37,071 4,13,93,997  +  AXIN1 protein_coding axin 1 [Source:HGNC Symbol;Acc:HGNC:903] − 3.20 1.35E−03
ENSSSCG00000039416 15 1,56,63,494 1,56,67,233  +  CXCR4 protein_coding C-X-C motif chemokine receptor 4 [Source:NCBI gene;Acc:396659] − 2.54 2.55E−02
ENSSSCG00000016925 16 3,72,99,116 3,73,05,116 PLK2 protein_coding polo like kinase 2 [Source:HGNC Symbol;Acc:HGNC:19699] − 2.38 4.91E−02
ENSSSCG00000022390 X 4,17,26,526 4,17,49,726  +  RGN protein_coding regucalcin [Source:HGNC Symbol;Acc:HGNC:9989] − 1.91 9.43E−03
ENSSSCG00000006562 4 9,57,59,073 9,58,64,458  +  GATAD2B protein_coding GATA zinc finger domain containing 2B [Source:HGNC Symbol;Acc:HGNC:30778] − 1.68 9.43E−03
ENSSSCG00000004390 1 7,50,33,098 7,51,69,987 SESN1 protein_coding sestrin 1 [Source:HGNC Symbol;Acc:HGNC:21595] − 1.62 1.67E−02
ENSSSCG00000026784 2 7,61,25,926 7,61,46,733  +  LMNB2 protein_coding lamin B2 [Source:HGNC Symbol;Acc:HGNC:6638] − 1.55 1.44E−02
ENSSSCG00000014267 2 13,37,08,266 13,39,51,438   protein_coding Rap guanine nucleotide exchange factor 6 [Source:NCBI gene;Acc:100521255] − 1.54 1.44E−02
ENSSSCG00000047299 AEMK02000489.1 43,232 45,101  +  RN18S rRNA 18S ribosomal RNA [Source:NCBI gene;Acc:100861538] − 0.71 3.89E−06
ENSSSCG00000012847 2 4,35,125 4,73,744  +  TALDO1 protein_coding transaldolase 1 [Source:NCBI gene;Acc:100514210] 0.85 4.90E−02
  1. aEnsembl (https://m.ensembl.org/index.html) gene ID
  2. bChromosome or scaffold number where gene is located
  3. cLog 2-fold change relative to ICSI-derived blastocyst
  4. dAdjusted p-value