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Fig. 4 | Epigenetics & Chromatin

Fig. 4

From: Mitochondrial supplementation of Sus scrofa metaphase II oocytes alters DNA methylation and gene expression profiles of blastocysts

Fig. 4

Methylation status of the imprinted gene IGF2R in Sus scrofa oocytes (Oc) and ICSI-derived (IB) and mICSI-derived (MB) blastocysts. Levels of CpG methylation were calculated using the 100-CpG probe method and displayed as bar histograms in SeqMonk. Red and blue dots above each histogram indicate methylated and unmethylated C counts, respectively. Triplicate BS-seq data for each sample type are shown. Gene and CGI annotated regions are shown at the top panel. DMRs between ICSI- and mICSI-derived blastocysts from the WGBS data were determined by three DMR callers, SeqMonk (sqm), methylKit (mk) and DSS (dss), and indicated by grey boxes under the genomic features at the top part of the panel. The genomic region corresponding to the imprint control region (ICR) identified in human and mouse is indicated by the red bar at the bottom of the panel

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Epigenetics & Chromatin

ISSN: 1756-8935