Fig. 5
Differentiation-state-specific methylation patterns parallel changes on chromatin accessibility occurring upon lens fiber cell differentiation. A Proportion of mCG DMRs that overlap a chromatin-accessible region in the lens genome. Opening chromatin indicates a chromatin-accessible region that is significantly more open in lens fiber cells compared to lens epithelial cells (ATACseq log2FC > 0, adjusted p < 0.05). Closing chromatin indicates the inverse (ATACseq log2FC < 0, adjusted p < 0.05). Stable/unchanged open chromatin indicates a chromatin-accessible region that does not change significantly between lens fiber cells and lens epithelial cells (ATACseq adjusted p > 0.05). This plot is limited only to mCG DMRs that overlap at least one chromatin-accessible region. χ2 test, p < 1 × 10–300. B Scatter plot of the change in methylation (diff. methyl) of mCG DMRs plotted against the change in chromatin accessibility at the same region. The plot is divided into significant changes in chromatin accessibility (ATACseq adjusted p < 0.05) and non-significant changes (adjusted p > 0.05). This plot is limited only to mCG DMRs that overlap at least one chromatin-accessible region. Pearson correlation r = − 0.86, p < 1 × 10–300