Fig. 4
Differentiation-state-specific methylation patterns parallel differentiation state-specific gene expression changes during lens fiber cell differentiation. A Scatter plot of average change in mCG methylation (Average diff. Methyl) of all mCG DMRs mapped to the promoter (− 2 kb upstream of transcription start site) and genebody versus the log2-fold change in gene expression of differentially expressed genes (DEGs) between lens fiber cells and lens epithelial cells. Data indicate only DEGs that contain at least one mCG DMR. Pearson correlation r = − 0.37, p < 1 × 10–42. B Proportion of DEGs (upregulated/fiber cell gene or downregulated/epithelial cell gene) that also have an increase in average methylation (more methylated in fiber cells compared to epithelial cells) or decrease in average methylation (demethylated in fiber cells compared to epithelial cells) across the promoter and genebody combined. Data indicate only DEGs that contain at least one mCG DMR. χ2 test, p < 1 × 10–43. C Same as A but only the average change in methylation of mCG DMRs mapped to putative promoters only. Data indicate only DEGs with putative promoters that contain at least one mCG DMR. Pearson correlation r = − 0.39, p < 1 × 10–11. D Same as B but only the average change in methylation of mCG DMRs mapped to putative promoters only. Data indicate only DEGs with putative promoters that contain at least one mCG DMR. χ2 test, p < 1 × 10–9