Fig. 5

Broad domains of H3K27ac in caudal sperm overlap with broad domains of H3K4me3 in MII Oocytes and colocalize to regions of open chromatin identified in the ATAC-seq profiles of MII oocytes and sperm. Venn diagrams comparing broad domains of H3K27ac identified in caudal sperm to broad domains of H3K27ac (A) and H3K4me3 (B) previously identified in MII oocytes (dataset GSE72784, [39]). Overlapping H3K27ac-H3K27ac (C) and H3K4me3-H3K27 (D) regions predominantly map to within 1 kb of gene promoters. E Correlation analysis between H3K9me2 and H3K27ac-enriched regions in caput and caudal sperm with regions of open chromatin identified using ATAC-seq in MII oocytes and sperm (GSE116854 [38]). Heatmaps showing the localization of broad (F) and narrow (G) H3K27ac peaks in caudal sperm at ATAC-seq Tn5-hypersensitive sites sperm and MII oocytes