Fig. 4

During epididymal maturation, broad H3K27ac peaks sharpen and transition from intronic and distal regions to gene promoters and enhancers driving embryo development. A Correlation analysis between H3K27ac ChIP-seq signals derived from sperm isolated from the caput and cauda regions of the epididymis (n = 2). Venn diagrams comparing changes in B broad and C narrow H3K27ac peaks between caput and cauda-derived sperm. D Integrative Genome Viewer tracks of chromosomal regions exhibiting the transition from diffuse broad peaks to more focused enrichment between caput and cauda-derived sperm. Bar chart representation of the distribution of H3K27ac across key genomic features between E caput and F cauda-derived samples. Heatmaps displaying the enrichment of H3K27ac signals across G CpG islands, H transcriptional start sites, or I regions with a proximal enhancer-like signature