Fig. 1

Gains and losses of select histone posttranslational modifications during epididymal maturation of mouse sperm. A Heatmap comparing the LC/MS-derived percentages for each peptide transition as a fraction of the total peptide pool (all modifications + unmodified peptides = 100%). Data represent the average of three independent runs obtained from a pooled sample of 10 million caput and cauda sperm. B–M Bar graphs contrasting gains and losses of unmodified (UN) histones with the levels of lysine acetylation (AC), monomethylation (ME1) dimethylation (ME2), and trimethylation (ME3) in sperm isolated from the caput and caudal portions of the epididymis. Error bars represent the SEM, n = 3 replicates, ** p < 0.01, *** p < 0.001, **** p < 0.0001