Fig. 2

HDAC inhibition alters linker histone H1 subtype expression in mESCs. A Reverse-phase HPLC (RP-HPLC) and Mass Spectrometry (inset) analysis of histone H1 subtypes in control and VPA-treated (2 mM, 48 h) mESCs. X axis: elution time [min]; Y axis: absorbency at 214 nm [mAU, milli-absorbency units]. The inset shows the relative signal intensity of H1d and H1e mass spectral peaks in the H1d/H1e fraction collected from HPLC eluates. Prominent changes are indicated in red arrows. B Quantification of H1/nucleosome ratios of individual H1 subtypes as well as of total H1 in control versus VPA-treated mESCs as determined by RP-HPLC and Mass Spectrometry. Data are expressed as the mean ± SD over three independent replicates. Unpaired t-test, two tailed; *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001