Fig. 3

Knockdown of Asf1a or Asf1b had a detrimental effect on developmental progression. A, B Representative images of embryos developed from Control-MO, Asf1a-MO, or Asf1b-MO microinjected zygotes. C, D Quantification of developmental progression. Data were obtained from three independent experiments and the total number of embryos used for the quantification was as indicated. (Control-MO, n = 98; Asf1a-MO, n = 107; Asf1b-MO, n = 137). E Immunofluorescence detection of Asf1a in 4-cell, morula and blastocyst stage embryos developed from Control-MO (left) or Asf1a-MO (right) microinjected zygotes. F, G Quantification of Asf1a fluorescence intensity in 4-cell (Control-MO, n = 44; Asf1a-MO, n = 44) and morula (Control-MO, n = 36; Asf1a-MO, n = 36). H Immunofluorescence detection of Asf1b in 4-cell, morula and blastocyst stage embryos developed from Control-MO (left) or Asf1b-MO (right) microinjected zygotes. I Quantification of Asf1b fluorescence intensity in 4-cell stage embryos. (Control-MO, n = 38; Asf1b-MO, n = 30). All quantification data were presented as mean ± SEM, and analyzed using Student’s t-test, *P < 0.05, **P < 0.01 and ***P < 0.001; DNA was stained with DAPI, Scale bar 20 µm