Fig. 1

Dynamic changes of the nuclear deposition of Asf1a and Asf1b in mouse oocytes and pre-implantation embryos. A, B Immunofluorescence staining for Asf1a (green) and Asf1b (green) in mouse oocytes at the germinal vesicle (GV), and pre-implantation embryos at the one-cell (1-cell), two-cell (2-cell), four-cell (4-cell), morula (morula) and blastocyst (blastocyst) stages. 9 to 22 embryos were analyzed in each group. DNA was stained with DAPI (blue). Scale bar, 20 µm. C, D Relative expression of histone Asf1a mRNA (C) and Asf1b mRNA (D) in mouse GV oocytes and pre-implantation embryos. The mean value for GV oocytes was set as 1, and relative values for all other samples were calculated accordingly. Data were obtained from three independent experiments and presented as mean ± SEM. Significant difference (a versus b, b versus c; P < 0.05) was determined by the one-way ANOVA and Student’s t-test