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Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: Ethionine-mediated reduction of S-adenosylmethionine is responsible for the neural tube defects in the developing mouse embryo-mediated m6A modification and is involved in neural tube defects via modulating Wnt/β-catenin signaling pathway

Fig. 3

Ethionine induced excessive cell apoptosis in E10.5 embryos and HT-22 cells. A The cell apoptosis was examined by TUNEL assay (green) in WT, ethionine, SAM, ethionine+SAM E10.5 embryos. Cell nuclei were stained with DAPI (blue). The percentage of PCNA-positive cells in each region is shown. Scale bars, 500 μm. B, C BCL-2, Cleaved Caspase-3 protein levels in WT, ethionine, SAM, ethionine + SAM embryos brain in E10.5 were evaluated via Western blot and quantified; β-actin levels were also evaluated to confirm equal loading (n = 3). Each experiment was carried out in triplicates ***p < 0.01 vs. control group. (D and E) BCL-2 and Cleaved Caspase-3 in HT-22 cells treated with ethionine and SAM. β-Tubulin was used as a loading control. Bar graphs for protein abundance were quantitative data from three independent experiments. F, G The cell apoptosis in the HT-22 cells treated with ehionine and SAM was analyzed by flow cytometry. H, I Cell apoptosis was detected by AO-EB double staining and quantified. The late apoptosis cells with bright red fluorescence in ethionine-treated groups were significantly increased compared with that in the control group and ethionine+SAM group. Scale bar: 100 μm

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Epigenetics & Chromatin

ISSN: 1756-8935