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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: Ethionine-mediated reduction of S-adenosylmethionine is responsible for the neural tube defects in the developing mouse embryo-mediated m6A modification and is involved in neural tube defects via modulating Wnt/β-catenin signaling pathway

Fig. 1

Ethionine induced neural tube defects by damaging the methionine cycle. A–D Ethionine-induced anencephaly in C57BL/6 mouse embryos at E10.5. Scale bars, 500 μm. E, F H&E staining results of normal and NTDs embryonic neural tube in E10.5. Scale bars, 100 μm. G The abundance of SAM was significantly lower in E10.5 NTDs embryos than in wild-types (*p < 0.05) SAH concentration (H) was elevated, and the SAM/SAH ratio (I) was reduced in ethionine-induced embryos compared with normal embryos (*p < 0.01, significantly differs from ethionine-induced embryos). However, the levels of SAM were increased significantly after treating with 2 mM SAM. H SAH and the SAM/SAH ratio (I) was reduced in ethionine-induced mouse embryos compared with normal embryos (**p < 0.01), which is significantly different from control. Similarly, after giving 2 mM SAM compensation, the levels of SAH and SAM/SAH ratio were obviously increased (*p < 0.05). n = 8 samples per genotype for each tissue

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Epigenetics & Chromatin

ISSN: 1756-8935