Fig. 6
From: Revealing RCOR2 as a regulatory component of nuclear speckles
RCOR2 regulates nuclear speckle morphology. A Western blot analysis of RCOR2 levels under RCOR2 knockdown in HeLa cells. GAPDH was assayed as a loading control. Panel is representative of three biological replicates. B Western blot analyses of nuclear speckle components after knocking RCOR2 down. GAPDH was assayed as a loading control. The blot includes two biological replicates per condition. C RT-qPCR results normalized to GAPDH as a constitutively expressed transcript in control and siRCOR2 cells. RCOR2 mRNA was analyzed with two different sets of primers targeting different RCOR2 exons. Results are representative of three biological replicates. ***p < 0.01. D SON immunostaining (magenta-hot) on HeLa cells transfected with siControl or siRCOR2 siRNAs. E SON immunostaining (magenta-hot) on HeLa cells transfected with pcDNA3.1 or pcDNA3.1-HA-RCOR2 plasmids. F–G Speckle area was measured after segmenting SON immunostainings from knockdown (F) and overexpression (G) experiments. Particle area was measured and the population values were grouped into quintiles. Each quintile was compared to its respective control group and statistical significance was tested. *p < 0.05. **p < 0.01. ****p < 0.001. H Circularity and elongation of speckles were measured on RCOR2 knockdown samples. Statistical significance was tested on the top 50% particles from each condition. Red lines represent median values. n.s: non significant. **p < 0.01. I Circularity and elongation of speckles were measured on RCOR2 overexpression samples. Statistical significance was tested on the whole population of particles from each condition. Red lines represent median values. **p < 0.02. ****p < 0.001