Fig. 5
From: Revealing RCOR2 as a regulatory component of nuclear speckles
RCOR2 behaves as a core component of nuclear speckles. A–D HeLa cells were stained against RCOR2 (green) and SRRM2 (red) after treatments with DMSO vehicle (A), actinomycin D (B), isoginkgetin (C) and tubercidin (D). Arrows indicate cytoplasmic SRRM2 aggregates. Images are representative of two independent experiments. E RCOR2 overlapping degree is expressed as the percentage of thresholded RCOR2 pixels overlapping SRRM2 territories. Percentages were calculated based on RCOR2 thresholded Manders coefficients analyzed by JACoP ImageJ-plugin on independent stainings. **p < 0.01. F Speckle area quantitation under isoginkgetin and actinomycin D treatments. The area was measured in 10 cells measuring areas of 10 speckles per cell on each condition. Results were normalized against DMSO control values. Unpaired t-tests were performed for each condition against mock-treated cells to estimate statistical significance. ****p < 0.001