RCOR2 colocalizes with nuclear speckles. A Different cell types immunolabeled for RCOR2 (green). B–F HT22 cells were stained with double immunolabeling of RCOR2 (green) and nucleophosmin (A), coilin (B), H3K9me3 (C), HP1α (D) or SRRM2 (E) as molecular markers of nuclear bodies or chromatin condensates (red). For each staining procedure, the spatial correlation of both fluorophores plus Hoechst staining was tracked according to the white dashed lines. Right-side plots show their normalized fluorescence intensity plotted against the position on the dashed line. Images are representative of three independent stainings. G RCOR2 overlapping degree is expressed as the percentage of thresholded RCOR2 pixels overlapping territories of red channel pixels. Percentages were calculated based on RCOR2 thresholded Manders’ coefficients analyzed by JACoP ImageJ-plugin on independent stainings. H HeLa cells were transfected with a plasmid to over-express recombinant HA-RCOR2. Cells were immunostained with an anti-HA epitope antibody (green). I Double immunolabeling of RCOR1 (red) and RCOR2 (green) on HT22 cells.