The methylome of Biomphalaria glabrata and other mollusks: enduring modification of epigenetic landscape and phenotypic traits by a new DNA methylation inhibitor

Table 5 Biomphalaria glabrata gene-specific primers used to amplify gene fragments used in the RT-qPCR

Gene	Primer Sequence	Amplicon length	Primer efficiency
28S ribosomal protein	F: GCTGGCACGACCGCTCCTTT	100 bp	2.01
28S ribosomal protein	R: TTTGAACCTCGCGACCCGGC	100 bp	2.01
α-Tubulin	F: CGACATCTGCCGCCGTAACCT	112 bp	2.04
α-Tubulin	R: GGCGCCATCAAACCTGAGGGA	112 bp	2.04
BGLTMP010125-RA:	F: TTGCTGTGACTGTCAGTGTC	95 bp	1.90
BGLTMP010125-RA:	R: TAGACTCAATGGACGGTGGAC	95 bp	1.90

ISSN: 1756-8935