Skip to main content
Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: Telomere-specific chromatin capture using a pyrrole–imidazole polyamide probe for the identification of proteins and non-coding RNAs

Fig. 5

PI-PRICh detects ALT cell-specific ncRNAs transcribed from around the inserted telomeric repeats. a ncRNAs mapped to the introns of SPOCK3 and USP16 genes in ALT cells. These ncRNAs were highly enriched in the TH59-DB fraction from ALT cells (lower, U2-OS), but not from telomerase-positive cells (upper, HeLa1.3). Red arrowheads indicate the positions of the inserted telomeric repeats. b Sequence analysis of DNA fragments captured by TH59-DB. Genomic regions coding the introns of SPOCK3 and USP16 were specifically enriched in TH59-DB pull-down fraction of U2-OS, but not in that of HeLa1.3. The positions of the inserted telomeric repeats are indicated by red arrowheads. c Representative sequence reads containing both telomeric repeats (yellow) and SPOCK3 (gray) or USP16 intron region (light blue) in TH59-DB pull-down fractions from U2-OS cells

Back to article page

Epigenetics & Chromatin

ISSN: 1756-8935