Fig. 3
Comprehensive identification of telomeric chromatin-associated RNAs in MEL cells. a The percentage (yellow) of telomeric repeat reads, including TERRA transcripts, in the input and TH59-DB pull-down fractions from MEL cells. The number of the single-end reads, including (TTAGGG)5 or (CCCTAA)5, extracted from input and TH59-DB pull-down fractions were divided by the total number of reads. The gray color shows the other reads. b Scatter plot of fragments per kilobase of per million mapped reads (FPKM) of the TH59-DB pull-down fraction versus that of the input sample for each RNA. The telomerase RNA component, TERC (most enriched in the TH59-DB pull-down fraction), is highlighted in blue. RNAs that enriched more than 100-fold in TH59-DB pull-down fraction are plotted below a red dotted line. c Bar graph of FPKM of telomerase RNA-component (TERC) in input and TH59-DB pull-down fractions. d Bar graph of FPKM value of the transcripts at a 30-kb region adjacent to the telomere on the q arm of each chromosome in input and TH59-DB pull-down fractions. e Sequence analysis of DNA fragments captured by TH59-DB. The percentage (yellow) of telomeric repeat reads including (TTAGGG)5 or (CCCTAA)5, in the input and TH59-DB pull-down fractions from MEL cells. Note that the results of the second experiment (Experiment 2) are shown in Additional file 2: Figure S2