Fig. 4

MORC3 regulates chromatin accessibility at ERVs. a Percentage of the total ATAC peaks (n = 11,000) that show increased chromatin accessibility (left panel) and heatmap showing the difference in chromatin accessibility between Morc3^MD41/MD41 and the WT (right panel, n = 2 biological replicates). Each row corresponds to chromatin accessibility of a single locus. b Barplot showing the annotation of the 298 differentially accessible loci (DAL). c Representative genome browser tracks showing a change in accessibility in Morc3^MD41/MD41 occurring at an LTR elements (RepeatMasker track) covered with H3K9me3. The DAL is shown by the black arrow. d Metaplot showing the ATAC-seq coverage at LTR, IAP, LINE and SINE in WT (red) and Morc3^MD41/MD41 (blue) with 1 kb flanking region. Replicates are shown in a different shade of the same color. e Metaplot showing the average H3K9me3 read coverage at all DAL. f Trim28 ChIP-qPCR at DAL in WT (red) and Morc3^MD41/MD41 (blue)