Fig. 1

Identification of a Morc3 allele from the MommeD screen. a FACS analysis and quantification of 10,000 erythrocytes from 3-week-old mice. Representative GFP profiles for MommeD41 WT and MommeD41 heterozygous mice (littermates) are shown. The MommeD41 allele demonstrates a higher percentage of erythrocytes expressing GFP and an increased mean fluorescence of expressing cells compared to WTs. Red line represents MommeD41 and black line represents WT. b Sanger sequencing trace of WT and Morc3^{MD41/ MD41} demonstrating a T to A transversion. c Timed matings and intercrosses show the number of embryos/mice observed (and in brackets the percentage) at the indicated timepoints. d Western blot validates the loss of MORC3 (~ 110 kDa) in Morc3^MD41/MD41 mESCs (n = 3, biological replicates). Tubulin (~ 50 kDa) is shown as a loading control. The higher (> 110 kDa) bands may represent sumoylated forms of MORC3, as previously shown by Mimura et al. [54]