Fig. 5

CpG methylation and gene expression patterns in the ventricles of juvenile snapping turtles. Probability density function for CpG methylation level in various genome features (A–F). A Intergenic regions (n = 12,840,311), B gene bodies (n = 3,426,421), C promoters (n = 68,168), D first exons (n = 40,226), E remaining exons (242,295), and F introns (n = 3,1561,179). Violin plots showing CpG methylation levels for various gene features as a function of gene expression level (G–K). Genes were divided into expression deciles with the lowest expressed genes in the first decile and the highest expressed genes in the tenth decile as shown on the X axis. The width of the violin corresponds to the probability density function for the methylation level shown on the Y axis. The bar in the middle of each violin shows the interquartile range and the white dot shows the median methylation level for each expression decile. Spearman’s rank correlations (ρ) between DNA methylation and gene expression were highly significant for all gene features (***p < 0.001). G Gene bodies (n = 3,426,421), H promoters (n = 68,168), I first exons (n = 40,226), J remaining exons (242,295), and K introns (n = 3,1561,179)