Fig. 10

Decreases in histone H4 acetylation and H3K4me3 levels in adult ovaries following gestational PB exposure. Exposure to PB affects histone H4Ac and H3K4me3 levels in grown oocytes in adult ovaries. A Representative images of control (top) and PB-treated oocytes (bottom) immunostained for MSY2 (oocyte marker, green) or H4Ac (red) (40X magnification). B Quantitative analysis of the H4Ac signals in oocytes and in the surrounding granulosa cells; n = 4 for each group; *p < 0.05, nonparametric Mann–Whitney test. C Representative images of antral follicles immunostained for H3K4me3 (red) and MSY2 (oocyte marker, green). D Quantitative analysis of H3K4me3 signals in granulosa cells and oocytes from control and PB-treated samples; n = 4 for each group; p** < 0.01, nonparametric Mann–Whitney test