Fig. 1

Meiosis defects are associated with increases in histone acetylation and H3K4me3. A Surface spreads from E18.5 ovaries from control (first row) and Pb-exposed mice (second row) were immunostained with anti-H3K9Ac (green) and anti-SYCP3 (red) antibodies (63X magnification). The dotted circles represent telomere connections. B Quantitative analysis of immunofluorescence intensity. The data are presented as the averaged fluorescence compared to the control ± SEM; n = 4 control, n = 4 PB group; *p < 0.05, nonparametric Mann–Whitney test. C Surface spreads from E185 ovaries from control (first row) and PB-exposed mice (second row) were immunostained with anti-H3K4me3 (green) and anti-SYCP3 (red) antibodies; the arrows represent telomere end-to-end connections. D Quantitative analysis of anti-H3K4me3 intensities. The analysis was performed on at least 20 oocytes from four replicates for each group; n = 4 for each group; *p < 0.05, nonparametric Mann–Whitney test