Fig. 4

Chromosome instability upon removal of chromosome 7 pericentromeric H3K9me3. A U2OS cells expressing the inducible TALE-demethylase. DNA is visualized using Hoechst, the α-satellite repeats of chromosome 7 and 11 (shown in orange and green, respectively) are labeled using specific LNA probes. B Bar plot showing the level of chromosome instability (percentage of nuclei with more or less than 4 chromosomes) in over 1000 nuclei for chromosome 7 (orange) or chromosome 11 (green) upon expression (light) or not (dark) of the TALE-demethylase (left) or of the TALE-GFP (right). p values were computed using a Chi-squared test (**p < 0.01). Scale bar, 5 µm