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Fig. 6 | Epigenetics & Chromatin

Fig. 6

From: The histone chaperone Nrp1 is required for chromatin stability and nuclear division in Tetrahymena thermophila

Fig. 6

Immunofluorescence staining of γ-H2A.X. Immunofluorescence staining of γ-H2A.X after completion of micronuclear meiosis. Post-meiotic DSBs triggers H2A.X phosphorylation. The unselected pronuclei retaining γH2A.X are eventually eliminated. A γ-H2A.X signal occurred in the meiotic MICs and disappeared in the selected pronuclei in WT cells. a/a′/a′′, post-meiotic γ-H2A.X formation; b/b′/b′′, pronuclear selection; c/c′/c′′, zygotic formation. B γ-H2A.X occurred and maintained in the meiotic MICs and disappeared with the MIC degradation in the nrp1i mutants. d/d′/d′′, post-meiotic γ-H2A.X formation; e/e′/e′′, pronuclear selection; f/f′/f′′, degradation of micronuclei. The arrows indicate the selected pronuclei. The stars indicate zygotes. Scale bar, 10 µm

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Epigenetics & Chromatin

ISSN: 1756-8935