Fig. 1
Characterization of NRP1 from T. thermophila. A Conserved domain of four different NASP family proteins. TPR indicated tetratricopepeptide repeats; D/E-rich residues indicated rich aspartic and glutamic acid regions. NLS indicated nuclear localization signal peptide. B Phylogenetic tree of NASP family proteins from different eukaryotic organisms reconstructed using TPR amino acid sequences. Tree topology and branch lengths correspond to Bayesian inferences. T.c (Tetrahymena canadensis Nrp1, TSP00244100); T.b (Tetrahymena borealis Nrp1, TBOREA00197080); T.e (Tetrahymena elliotti Nrp1, TELLIO00222160); T.t (Tetrahymena thermophila Nrp1, XP_001030823.3); T.p (Tetrahymena pyriformis Nrp1, TPYRIF00252750); T.em (Tetrahymena empidokyrea Nrp1, TEPIDO00181820); I.m (Ichthyophthirius multifiliis Nrp1, XP_001030823.3); R.n (Rattus norvegicus NASP, NP_ 001,005,543.1); M.m (Mus musculus NASP, NP_058057.3); C.g (Cricetulus griseus NASP, XP 007,609,458.1); H.s (Homo sapiens NASP, XP_005270945.1); M.n (Macaca nemestrina NASP, XP_011762308.1); P.a (Pongo abelii NASP, PNJ21692.1). P.x (Plutella xylostella NASP, XP_011559258.1); H.a (Helicoverpa armigera NASP, XP_021184247.1); P.m (Papilio machaon NASP, XP_014366859.1); B.m (Bombyx mandarina NASP, XP 028,027,096.1:26–395). B.n (Brassica napus Sim3, XP_013742238.1); R.s (Raphanus sativus Sim3, XP 018,450,125.1); A.l (Arabidopsis lyrata subsp Sim3, XP 020,874,858.1); E.s (Eutrema salsugineum Sim3, XP 006,411,948.1); M.d (Malus domestica Sim3, XP 008,377,655.2); M.e (Manihot esculenta Sim3, XP 021,632,704.1). D.f (Debaryomyces fabry Sim3, XP_015465497.1); S.p (Schizosaccharomyces pombe Sim3, NP_595313.1); C.z (Cercospora Zeina Sim3, XP_015465497.1); A.c (Aspergillus cristatus Sim3, ODM15912.1); P.i (Penicillium italicum Sim3, KGO72320.1); E.g (Elaphomyces granulatus Sim3, OXV08410.1); T.m (Talaromyces marneffei Sim3, KFX53481.1). C Expression profiles of the NRP1. The gene fragments were amplified by qRT-PCR at the vegetative stage (V), starvation stage (S), and sexual development (3 h, 5 h, 7 h, 9 h, 11 h, 13 h) stage. D The structure of Nrp1 was established with SWISS-MODEL