Fig. 7

Markers of caspase-initiated chromatin condensation. A Western blot analysis of γ-H2A.X, H2A.X pY142, H2B pS14, H2B K15Ac, H4 K16Ac post-translational marks in WT (n = 3) and in BAZ1B KO HUDEP-2 subclones (KO1–KO3) (n = 2) during maturation. HSC70 was used as loading control. B Western blot analysis of full-length Caspase-3 protein expression in WT (n = 3) and BAZ1B KO (n = 6) HUDEP-2 subclones at D0, D4, and D7 of maturation. HSC70 was used as loading control. The bar graph shows quantification as performed using ImageJ. Error bars represent ± SEM. Asterisks (*) indicate significance as follows: **p ≤ 0.01. p-values were calculated with a two-tailed Student’s t-test