Fig. 4

Markers of caspase-initiated chromatin condensation and apoptosis during erythroid maturation. Western blot analysis of H2B pS14, H2B K15Ac, and H4K16Ac expression during A HUDEP-2 (n = 3) and B human CD34+ progenitor (n = 2) maturation along with Caspase-3 protein expression. HSC70 was used as a loading control. Quantification was performed using ImageJ, with signal obtained at the earliest time point measured set at 1.0. p: phosphorylation, Ac: acetylation, RPL: relative protein level. C Schematic and graphical representations of flow cytometric analysis of Annexin-V and 7-AAD in human CD34+ progenitor cell cultures (n = 5 technical replicates of 2 biological replicates each). Red boxes indicate apoptotic cells. Error bars represent ± SEM. Asterisks (*) indicate significance as follows: **p ≤ 0.01; ****p ≤ 0.0001. p-values were calculated with a two-tailed Student’s t-test