Fig. 3

Transcriptome analysis of HUDEP-2 histone H2A.X KO cells. Heat map depicting differentially expressed genes (log2-fold change ≥ 1.5 and P-Value ≤ 0.001) in WT (n = 2) and H2A.X KO (n = 3) HUDEP-2 cell cultures at D0 (A) and D6 (B) of maturation. Bar graphs showing number of upregulated and downregulated genes relative to WT in H2A.X KO HUDEP-2 cell cultures at D0 (C) and D6 (D) of maturation. E Log2 values of fold-change for specific erythroid and hematopoietic genes at D0 and D6 of maturation. Green bar represents genes that typically are upregulated during erythropoiesis and the red bar represents genes that are typically downregulated during erythropoiesis. Asterisks (*) indicate significance as follows: *p ≤ 0.05; **p ≤ 0.01; ****p ≤ 0.0001