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Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: Histone H2A.X phosphorylation and Caspase-Initiated Chromatin Condensation in late-stage erythropoiesis

Fig. 2

Maturation of H2A.X knockout HUDEP-2 cell lines. A Illustration of CRISPR/Cas9 design used to generate H2A.X KO HUDEP-2 cell lines. B Western blot analysis of H2A.X protein expression in WT and H2A.X KO HUDEP-2 cell lines. HSC70 was used as a loading control and quantification was performed using Image J. WT: wild type, KO: knock out. n = 3. C Graph of WT and H2A.X KO cell expansion across a HUDEP-2 maturation time course, as measured by Trypan exclusion. D Representative Wright–Giemsa staining of WT and H2A.X KO HUDEP-2 cells during expansion (D0), and during D4 and D7 of maturation. Arrows indicate H2A.X KO cells that have not condensed their nuclei to the same degree as WT. E Analysis of hemoglobin accumulation, as measured by benzidine staining, in WT and H2A.X KO HUDEP-2 cells from D1–D4 of maturation. Error bars represent ± SEM in B & E and ± SD in C. Asterisks (*) indicate significance as follows: **p ≤ 0.01; ***p ≤ 0.001. P-values were calculated with a two-tailed Student’s t-test

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