Fig. 1

Presence of histone H2A.X and γ-H2A.X during terminal erythroid maturation. Imaging (A) and quantification (B) of γ-H2A.X foci in sorted murine bone marrow (n = 4). Giemsa staining of WT (C) HUDEP-2 cells at D0, D4, and D7 of maturation and D WT human CD34+ progenitor cells at D6, D8, and D10 of maturation after undergoing CD36 synchronization. Western blot analysis of histone H2A.X, γ-H2A.X (H2A.X pS139), and H2A.X pY142 during maturation in E HUDEP-2 (n = 3) and F human CD34+ progenitor (n = 2). HSC70 was used as a loading control. Quantification was performed using ImageJ. Signal obtained at the earliest time point measured was set at 1.0. Error bars represent ± SEM. Asterisks (*) indicate significance as follows: *p ≤ 0.05; **p ≤ 0.01. p-values were calculated with a two-tailed Student’s t-test