Fig. 1

Identification of proximal interactors of DOT1L via BioID. a Schematic of BirA*-DOT1L fusion protein-expressing vector constructs. b H3K79 di-methylation (H3K79me2) levels in control (gControl) and DOT1L knock-out (gDOT1L) cells expressing either WT or MUT BirA*-DOT1L fusion constructs. Total histone H3 was used as a loading control. c Proximal-protein interactions of DOT1L as revealed via proteomic analyses of biotinylated proteins. Proteins were ordered according to their average coverage and PSM (peptide spectrum matches) values in BirA*-DOT1L wt expressing cells. Asterisk indicates proteins detected only in wt-DOT1L samples. d Schematic of shRNA-mediated somatic cell reprogramming timeline. e Bar graph represents fold change in reprogramming efficiency upon shRNA-mediated gene silencing. Tra-1-60 positive colony numbers of each experiment were normalized to shControl sample. Average of fold changes from independent experiments are indicated (circles). Representative Tra-1-60 stained well images for each shRNA-infected sample are displayed under the bar graph. Error bars represent SEM. *P < 0.05