Fig. 5

No change in habitual activity associated with MMS. In-cage behavioural monitoring using the TSE PhenoMaster system tracked movement over a 24-h period (two consecutive 24-h periods were averaged for each animal). A Cumulative distance travelled with respect to time of day, the light aspect of the graph indicates when the lights were on and the dark aspect indicates when the lights were off. B Total movement calculated for the light and dark phase (indicated by AM and PM, as well as the light and dark aspects) revealed no difference between the groups (p = 0.25, f = 1.40, df = 24) (n = 7/group) and no interaction with time (p = 0.38, f = 0.79, df = 24). As expected, there was, a significant effect of time such that animals were active during the dark phase (p = 0.003, f = 10.57, df = 24). A two-way ANOVA with repeated measures was used to assess statistical associations in B. Error bars indicate standard error of the mean