Fig. 2
From: Altered hypothalamic DNA methylation and stress-induced hyperactivity following early life stress
3’ mRNA sequencing of the hypothalamic transcriptome reveals only subtle changes associated with MMS. 6 samples were sequenced per group. A Volcano plot showing differential gene expression between groups, genes with a logFC greater than 1.5 are coloured blue (decrease) or red (increase). Only 1 gene (D630033O11Rik) had an FDR > 0.05. B Gene Ontology analysis of genes with a logFC > 1.5 (i.e. those coloured blue or red in A) for cellular component (pink), biological process (yellow) and molecular function (green). C Gene expression patterns, from control (clear circles) and MMS (black filled circles), identified from 3’ mRNA sequencing and Gene Ontology were validated using qPCR. FOS was enriched in the Gene Ontology term “Ligand-gated cation channel activity” and NR4A3 was identified from the enriched term “DNA-binding transcription activator activity, RNA polymerase II-specific”, in the biological process category. Both FOS (p > 0.001, df = 17) and NR4A3 (p = 0.004, df = 16) were significantly increased in expression following MMS