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Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: Programmed suppression of oxidative phosphorylation and mitochondrial function by gestational alcohol exposure correlate with widespread increases in H3K9me2 that do not suppress transcription

Fig. 5

In vitro analysis of alcohol-induced changes in histone post-translational modifications. Primary fetal cerebral cortical neuroepithelial stem cells were cultured in the presence of 160 or 240 mg/dL EtOH for 3 days, followed by a 4-day recovery in medium lacking EtOH. A Examination of transcripts encoding candidate genes within the Oxidative Phosphorylation, Mitochondrial Dysfunction, and EIF2 Signaling pathways in RNA samples isolated from treated neuroepithelial stem cells. Gene expression was normalized to transcripts encoding Gapdh and Ywhaz (n = 4). We assayed cellular extracts isolated from treated neuroepithelial stem cells for the enrichment of B histone h3, lysine 9 dimethylation (H3K9me2) C histone h3, lysine 9 trimethylation (H3K9me3), and D histone h4 lysine 20 trimethylation (H3K20me3). ChIP-qPCR primers assayed the enrichment of the post-translational modifications within an untranscribed region of chromosome 6 (Untr6) and the indicated transposable elements. Enrichment of E H3K9me3 and F H4K20 within the regulatory region of Nkx2.1. For ChIP-qPCR analysis n = 4. Error bars represent the SEM, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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Epigenetics & Chromatin

ISSN: 1756-8935