Skip to main content
Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: Programmed suppression of oxidative phosphorylation and mitochondrial function by gestational alcohol exposure correlate with widespread increases in H3K9me2 that do not suppress transcription

Fig. 3

Increases in histone H3, lysine 9 dimethylation do not associate with candidate gene transcriptional silencing. A RT-qPCR validation of the top upregulated genes in the cortices of control, EtOH-exposed unaffected, and EtOH-exposed affected fetal mice. Gene expression was normalized to transcripts encoding Gapdh and Ywhaz. (n = 7). B Using chromatin immunoprecipitation qPCR (ChIP-qPCR), we assayed the enrichment of histone H3, lysine 9 dimethylation (H3K9me2) within the regulatory regions of upregulated and downregulated candidate genes. C Transcript levels of previously identified candidate genes exhibiting a > 500% increase in the enrichment of H3K9me2 within their regulatory regions. *P < 0.05, **P < 0.01, ***P < 0.001

Back to article page