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Fig. 3 | Epigenetics & Chromatin

Fig. 3

From: MMP-2 is a novel histone H3 N-terminal protease necessary for myogenic gene activation

Fig. 3

Myogenic gene activation is dependent on MMP-2. a RT-qPCR analysis of total RNA purified from control and C2C12 cells transiently transfected with a non-specific siRNA (siNS) or MMP-2-specific siRNA (siMMP-2) and cultured in differentiation media for 2 days. qPCR was performed for MMP-2 and the indicated myogenic genes (x-axis), normalized to the 18S housekeeping gene and plotted relative to C2C12 control for each gene (y-axis). Three independent biological replicates were performed to generate standard deviation (error bars). The Student’s t test was used to determine statistical significance between control and siMMP-2: p < 0.01 (*) and p < 0.03 (**). b Gelatin zymography of recombinant MMP-2 and cultured media collected from control, siNS and siMMP-2 day 2 C2C12 cells (top). Western analysis to detect the H3cl product in purified chromatin (middle). Amido black staining (AB) of membranes was performed to confirm equivalent loading of chromatin between samples (bottom). c RT-qPCR analysis as described above for control, siNS and siMMP-2 C2C12 cells cultured in differentiation media for 4 days. d Gelatin zymography and Western analysis as described above for control, siNS and siMMP-2 C2C12 cells cultured in differentiation media for 4 days

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