Skip to main content
Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: Dynamics of cattle sperm sncRNAs during maturation, from testis to ejaculated sperm

Fig. 2

Changes in sncRNA expression along the male reproductive tract. a Relative expression of each sncRNA class was computed for each of the five regions as the proportion (%) of the class expression relative to the total expression in the region: PAR (testis parenchyma), CAP (caput), COR (corpus), and CAU (cauda) epididymis and ejaculated sperm (SPZ). The bar graph clearly illustrates opposing trends between piRNAs, whose proportion decreases from parenchyma (78%) to cauda (13%) and ejaculated sperm (18%), and miRNAs and tsRNA, which follow an overall upward trend from a few % in parenchyma to 36% and 16% in ejaculated sperm, respectively. b Normalized expression level of each sncRNA class along the male tract. The proportion of total expression in each sampling regions is indicated above each bar for each sncRNA class (e.g., PAR and SPZ accounts for 2% and 36% of miRNA expression, respectively). c Nucleotide frequency at each position for all detected piRNA in each region. For each region, the frequency of each nucleotide was computed along the piRNA sequence and plotted as bar charts (from the 1st nucleotide at 5ʹ end to the 35th nucleotide). More than 75% of piRNA were shown to possess a U residue at their 5ʹ end (U1), and no enrichment for an A residue at position 10 (A10) was observed. d Relative expression of U1 relative to non-U1 piRNAs (V1) as well as A10 relative to non-A10 (B10) piRNA was computed per region. U1 piRNAs generated via the primary production pathway account for the majority of piRNA expression except at COR and CAU where piRNA expression is dominated by non-U1 piRNAs. Likewise, A10 piRNAs account for about 25% of piRNAs, except at COR where A10 piRNAs account to 60% of expression. B: IUPAC degenerate base symbols for not A, and V for not U

Back to article page