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Table 1 Mycelium growth at different temperatures

From: Loss of EZH2-like or SU(VAR)3–9-like proteins causes simultaneous perturbations in H3K27 and H3K9 tri-methylation and associated developmental defects in the fungus Podospora anserina

 

11 °C

27 °C

35 °C

WT

100

100

100

ΔPaKmt1

72.4

94.2

91.8

ΔPaKmt1 PaKmt1+

104

100

99.7

ΔPaKmt6

ND

Irregular slow growth

Irregular slow growth

ΔPaKmt6 PaKmt6+

98.3

99.0

99.3

ΔPaKmt1 ΔPaKmt6

ND

ΔPaKmt6 aggravated

ΔPaKmt6

ΔPaHP1

65.7

76.2

74.9

ΔPaHP1 PaHP1+

99.1

101

98.5

ΔPaKmt1 ΔPaPH1

60.7

89.9

80.7

ΔPaPH1 ΔPaKmt6

ND

ΔPaKmt6 aggravated

ΔPaKmt6

ΔPaKmt1 ΔPaPH1 ΔPaKmt6

ND

ΔPaKmt6 aggravated

ΔPaKmt6

  1. Relative growth rates (WT %) at optimal temperature (27 °C) and sub-optimal temperature (11 °C and 35 °C) on M2 minimal medium. ΔPaKmt6 mutant strains display irregular and slow growth at 27 °C and 35 °C, which cannot be quantified. At 11 °C, growth is too weak to be assayed (ND). At 27 °C, double and triple mutants containing the ΔPaKmt6 allele display aggravated ΔPaKmt6-like growth defects