Fig. 7

Chromatin modifications in the ΔPaHP1 strain. a Subcellular localization of PaHP1. Fluorescence microscopy pictures show PaHP1-GFP chimeric protein during vegetative growth in mycelium. Nuclei are marked using the PaH1-mCherry allele that encodes histone H1 protein tagged with mCherry fluorophore (red). Indicated scale is the same for all the pictures (5 µm). PaHP1-GFP displays nuclear localization and forms foci that likely correspond to heterochromatin domains (arrows). This specific localization is disturbed only in ΔPaKmt1 mutants. b Visualization of histone mark localization across the genome in the ΔPaHP1 strain. Domainograms (top) show significance of enrichment of H3K4me3, H3K9me3 and H3K27me3 marks in windows of varying size, according to the indicated p-value (right). ChIP-seq patterns (bottom) display histone modification coverage and MACS2 detected peaks. Both patterns are represented with the same scale on chromosomes one and five of P. anserina. Both centromeres are depicted (pink). The “Mat region” (orange) corresponds to the 800 kbp region devoid of recombination and containing the mating-type locus [93]. Repeated sequences are depicted in light blue. c Peak size distribution in the ΔPaHP1 mutant strain. Box plots showing sizes in base pair of the MACS2-predicted peak of H3K4, H3K9 and H3K27 trimethylation are shown in green, red and blue, respectively. The box is delimited, bottom to top, by the first quartile, the median and the third quartile. Numbers on top of each box represents the number of peaks detected. Outliers are not shown