Fig. 5

Gene expression in the ΔPaKmt6 strain and growth features of the ΔPaKmt6 and ΔPaKmt1 strains. a Relative expression of selected genes in ΔPaKmt6 mutant strains. Upper panel: histone marks in the wild-type strain. For each selected gene, a black dot shows the presence of the mark. Conversely a white dot shows its absence. The error bars represent the 95% confidence interval. Pa_4_1170 and Pa_1_16300 encode two putative Glycoside Hydrolases from families 7 and 61, respectively [51]. Pa_6_7270 and Pa_6_7370 belong to a secondary metabolite gene cluster located on a chromosome 7 sub-telomeric region. Pa_5_10 encodes the meiotic drive element Spok2 located on Chromosome 5 [56]. Pa_1_1880 encodes the putative DNA repair RAD50 protein. Pa_7_9210 encodes a putative exonuclease. They are both readily expressed at all stages of the life cycle and their expression remained unchanged in both of the two mutant backgrounds. Middle panel: normalized expression ratio of the selected genes relative to the wild-type strain. Overexpression (fold change ≥ 2, dark black line): Pa_1_6263: expression ratio = 25,987, p-value = 0.001; Pa_4_1170: expression ratio = 6375, p-value = 0.004; Pa_1_16300: expression ratio = 7588, p-value = 0.003; Pa_6_7270: expression ratio = 5280, p-value < 0001; Pa_6_7370: expression ratio = 6016, p-value = 0.002; Pa_5_10: expression ratio = 2212, p-value < 0.001; Pa_1_1880: expression ratio = 1.216, p-value = 0.192; Pa_7_9210: expression ratio = 0.756, p-value = 0.061. Three normalization genes (AS1, GPD and PDF2) were selected with geNorm [105] among eight housekeeping genes. Average expression stability of these three normalization genes is M = 0.230 and their pairwise variation is V3/4 = 0.071. See “Methods” for details and Additional file 22: Table S4 for Cq and selection of normalization genes and NRT-qPCR controls. Lower panel: histone marks in the ΔPaKmt6 mutant strain. They are indicated as in the upper panel (see above). b Quantification of expression of two Tc1_mariner-like members in ΔPaKmt1 and ΔPaKmt6 mutant strains. ΔPaKmt1 genetic context: no overexpression (fold change ≥ 2, dark black line). ΔPaKmt6 genetic context: Tc1_mariner-like_pelobates family: expression ratio = 7.4, p-value = 0.009 in ΔPaKmt6 genetic context and of Tc1_mariner-like_rainette family: expression ratio = 2.005, p-value = 0.004 in ΔPaKmt6 genetic context. Normalization as described in a. c Phenotypic characterization of ΔPaKmt1 and ΔPaKmt6 single mutant strains. The mat+ strains of the indicated genotypes were grown on M2 minimal medium for 5 days at 27 °C. d Vegetative growth kinetics of ΔPaKmt1 and ΔPaKmt6 single mutant strains on M2 minimal medium. See “Methods” section for details