Fig. 4

H3K36me2&3-associated transcriptional repression is largely independent of other regulatory mechanisms. a The H3K36me2&3-enriched and repressed gene set identified here for stage II gametocytes was compared to those previously determined to be repressed by HP1-mediated heterochromatin formation (+ HP1, gold) or by AP2-G2 (blue) in the stage II/III gametocytes. Certain genes from each of these categories are exemplified in the gene expression heatmaps [36]. b Log₂-transformed ChIP/input ratio tracks of H3K36me2 (turquoise) and H3K36me3 (purple) occupancy for jmjc1, hp1 and ap2-g in pre-stage II, stage II and post-stage II gametocytes, with the coding sequences of each represented by the green, maroon and orange blocks, respectively. Other genes are indicated by grey blocks. Dashed grey boxes represent regions of differential occupancy between the three stages and horizontal black bars indicate called peaks representing regions associated with H3K36me2&3 enrichment in stage II gametocytes. Full descriptions of gene names and their PlasmoDB IDs are available in Additional file 2: Table S4