Fig. 5
From: Early-life undernutrition induces enhancer RNA remodeling in mice liver
A novel enhancer RNA (eRNA) regulates the expression of core circadian gene Cry1. a IGV snapshot of sequencing data, identified novel enhancer on plus and minus strands, H3K27ac peaks, and RNA-seq data of Cry1. This novel enhancer (chr10: 85198257–85199349) regulates Cry1 expression directly. b Chromatin immunoprecipitation (ChIP)-Q-PCR assays for recruitment to Cry1 eRNA transcription site in NCD2 and PRD2 using ideal p300 antibody. Cry1-eRNA-1 and Cry1-eRNA-2 are two different primers for Cry-eRNA transcription site on the chromatin. c A key gene regulatory relationship between Cry1 and its downstream genes. Black arrow shows up-regulated RNA level detected in this study. d The high-throughput GRO-seq and RNA-seq data were calculated in column chart