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Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: Mechanism and functional role of the interaction between CP190 and the architectural protein Pita in Drosophila melanogaster

Fig. 5

Testing the role played by the CP190-binding region of Pita to induce an “open” chromatin structure on a polytene chromosome model. The left panel demonstrates the polytene chromosomes in phase contrast. The right panel is an overlay of phase contrast and immunostaining with antibodies against to Gal4 (red), Chriz (green), and CP190 (green). a Targeting the 94–285 aa Pita region (Pita) fused with the GAL4 DNA-binding region (GAL4DBD) to the 16 GAL4 binding sites in the 10A1-2 disc. At the top, the recruitment of GAL4DBD did not induce the formation of the interband in the 10A1-2 band (negative control). At the bottom, the recruitment of the 94–285 aa Pita region fused with GAL4DBD (G4(DBD)Pita) resulted in interband formation inside the band, and Chriz and CP190 proteins are detected in the decompacted area (shown in brackets and arrows). b The recruitment of chimeric proteins featuring the deletion of CP1 (G4(DBD)PitaΔCP1) or CP1 + CP2 (G4(DBD)PitaΔCP1+2) regions to the 10A1-2 did not induce interband formation inside the disc. The absence of CP190 and Chriz protein recruitment was detected simultaneously with the presence of a signal for Gal4 at the compact disk structure (red arrow)

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