Fig. 3
From: Molecular and computational approaches to map regulatory elements in 3D chromatin structure
Methods to map chromatin interactions. Simplified protocols of methods to map chromatin interactions are shown. DNA is first crosslinked, and fragmented with restriction enzymes (3C, 4C, 5C, Capture-C, Hi-C), DNaseI (DNase Hi-C), or MNase (Micro-C). After fragmentation, biotin is added for all methods except for 3C, 4C, 5C or Capture-C. The DNA then goes through proximity ligation, and reverse-crosslinked. Purification and amplification steps are followed. ChIA-PET and HiChIP use an antibody specific to TF or histone modification to map chromatin interactions associated with the specific TF or regulatory elements. IP: Immunoprecipitation