Fig. 2

Influence of hydrogen peroxide exposure on cell viability and percentage growth of synchronized cells. a Cells were exposed to two doses of H₂O₂, at both 2 and 3-h post-release. Each dose was at the stated concentration on the x-axis and viability was assessed after 24 h. Data are means and SE of three independent experiments. b Viability was assessed by flow cytometry based on the exclusion of PI at 4, 24, 48 and 72 h post-release. c The percentage growth (y-axis) for each replicate at 24, 48 and 72-h post-release (x-axis). Live cell counts conducted at 24, 48 and 72-h post-release were compared to the previous day's counts and the percentage growth calculated ((current day count—previous day count)/previous day count × 100). Circles represent control samples and triangles represent treatment samples. Data are means and SE of four independent experiments. Significant differences were determined with paired t-tests and are denoted with asterisks * = p < 0.05